Vatsayana Kamasutra Download Vatsayana Kamasutra. The First Book on Sex in the Sanskrit Tradition. Click on the button to download the PDF file of Kamasutra, which you can edit and print it yourself.Simian immunodeficiency virus (SIV) is a lentivirus closely related to human immunodeficiency virus (HIV). The central hypothesis of this proposal is that there is a critical interaction between cellular protein kinases and the viral protein regulatory protein, Tat, in determining the efficiency of gene expression in the host cell, both in vitro and in vivo. The first specific aim of this proposal will investigate the role of the cellular protein kinase, cyclin dependent kinase 1, in Tat-mediated transactivation of the viral LTR. We hypothesize that one of the functions of cyclin dependent kinase 1 in T cells is to be important in Tat-mediated transactivation of the LTR of HIV-1. This may be accomplished by either the direct phosphorylation of Tat or a cellular factor that is essential for the transactivation function of Tat. To test this hypothesis we will create mutant forms of cyclin dependent kinase 1 that either bind specifically to the LTR or inhibit the activity of the kinase. We will also generate mutant forms of cyclin dependent kinase 1 that are insensitive to Tat-mediated transactivation. These will be used to determine the role of cyclin dependent kinase 1 in the LTR and in transactivation. The second specific aim of this proposal will identify the protein(s) that bind to the viral transactivation region of Tat. We will identify this cellular protein(s) by in vitro selection of specific DNA binding proteins from a cDNA expression library. The cellular binding proteins will be identified by microsequencing. We will also identify the nucleotide sequence elements in Tat that are important in protein binding. The third specific aim of this proposal will determine if there is any host protein kinase that directly phosphorylates Tat and whether there is any inhibition of this phosphorylation when the cell is infected with a Tat mutant virus. To test these hypotheses we will purify cellular kinase from cells infected with HIV-1 or an HIV-1 mutant that lacks Tat. This will be accomplished by immunoprecipitation with a specific antibody to the cellular kinase and anion exchange chromatography. The purified kinase will be used to phosphorylate synthetic peptides of Tat and to phosphorylate Tat in infected cells be359ba680
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